General Cell Based Assays
Eurofins Discoverx PathHunter eXpress GPR157 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0414E2ACP2L)
The PathHunter GPR157 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR157 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR157 and EA-tagged β-Arrestin-2. Activation of GPR157 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress PTGER4 HEK 293 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0304E1CP0M)
The PathHunter PTGER4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. prostaglandin E2) based activation of PTGER4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PTGER4 and EA-tagged β-Arrestin-2. Activation of PTGER4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Arbor Assays Creatinine Serum Low Sample Volume Kit (384-well Plate)
The DetectX Low Volume Serum Creatinine kit quantitatively measures creatinine present in serum samples using a 384-well plate format to minimize sample usage.
Aat Bioquest iFluor 647 PSA Imaging Kit with Goat Anti-Mouse IgG (100 Tests)
Power Styramide Signal Amplification PSA system is one of the most sensitive methods that can detect extremely low-abundance targets in cells and tissues with improved fluorescence signal 10-50 times higher than the widely used tyramide TSA reagents. This catalog is for 100 Tests.
Aat Bioquest Screen Quest Membrane Potential Assay Kit Orange Fluorescence (1 plate)
Membrane potential is the difference in voltage between the interior and exterior of a cell. This catalog is for 1 plate.
Sigma Aldrich Fine Chemicals Biosciences 7-Aminoactinomycin D ~97% (HPLC), powder | 7240-37-1 | MFCD00077295 | 5MG
7-Aminoactinomycin D ~97% (HPLC), powder | Purity: ~97% (HPLC) | Mol Wt: 1270.43 | 7240-37-1 | MFCD00077295 | 5MG
Rockland Immunochemicals Sheep Hemagglutination Kit
10X Phosphate Buffered Saline (PBS) is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration which is 1X for this kit.SHEEP RED BLOOD CELLS (RBC) 10% Washed Pooled Cells - R405-0005 should be diluted in 1X PBS to 0.05% concentration.Anti-SHEEP Red Blood Cell (RBC) (RABBIT) Antibody - 213-4139-0002 binds and thus hemagglutinate Sheep RBCs when used at a dilution range of 1:25-1:50. An antibody mediated hemagglutination control is useful as a qualitative index for the integrity of the RBCs in the assay. Further serial dilutions up to 1:400 can be included in the assay if an independent or antibody-based control is required in the assay.
Sigma Aldrich Fine Chemicals Biosciences HMG CoA Reductase Assay Ki
The HMG-CoA Reductase Assay Kit is an important tool for the basic research of cholesterol and other relelated metabolic pathways.
Raybiotech Inc COVID-19 Spike-ACE2 Binding Assay Kit
The RayBio COVID-19 Spike-ACE2 binding assay kit is a rapid, simple, and sensitive method to characterize the binding affinity of the SARS-CoV-2 Spike (S) protein and the Angiotensin I Converting Enzyme 2 (ACE2) receptor complex in the presence of potential inhibitors.
Biolegend Alexa FluorR 647 anti-beta-act
Alexa Fluor 647 anti-β-actin [2F1-1]; Isotype: Mouse IgG2b, κ; Reactivity: Human, Mouse, Rat; Apps: IHC-P; Size: 100 μg
Biolegend Alexa FluorR 594 anti-beta-act
Alexa Fluor 594 anti-β-actin [2F1-1]; Isotype: Mouse IgG2b, κ; Reactivity: Human, Mouse, Rat; Apps: ICC, IHC-P; Size: 100 μg
Montana Molecular Borealis Arrestin Assay - B2AR D2040G, CMV, 10mL
Use the B2AR Borealis arrestin assay to measure arrestin signaling kinetics with an unmodified B2AR receptor. Add the receptor and fluorescent sensor to cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. Compare with cAMP signaling responses using Montana Molecular’s cADDis cAMP assay. These assays are genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells. Each kit includes the sensor, receptor, GRK, and positive control agonist.
Cell Biolabs Inc Glutamate Assay Kit (Fluorometric), 200 assays
Excess glutamate levels in the brain can cause cell injury and death, leading to neurological diseases. Cell Biolabs' Glutamate Assay Kit is a quantitative, fluorometric assay that uses glutamate specific enzymes to generate hydrogen peroxide. Glutamate levels as low as 300 nM can be detected in serum, plasma, urine, cell lysates, and cell culture supernatants. Each kit provides sufficient reagents for 200 assays including standards and unknown samples.
Cell Biolabs Inc CytoSelect™ 48-Well Cell Contraction Assay Kit (Floating Matrix Model), 48 assays
Several culture models exist to study fibroblasts' ability of to reorganize and contract collagen matrices in vitro. In the floating matrix contraction model, a freshly polymerized collagen matrix containing cells is released from the culture dish and allowed to float in culture medium, and contraction occurs in the absence of external mechanical load and without appearance of stress fibers in the cells. The CytoSelect™ 48-Well Cell Contraction Assay Kit provides a simple system to assess cell contractivity in vitro and screen cell contraction mediators using the floating matrix model. The kit's proprietary Cell Contraction Plate eliminates the matrix releasing step of the conventional contraction assay, providing a faster, higher-throughput method to assess cell contraction. Each kit provides sufficient quantities to perform up to 48 assays in a 48-well plate.